New York Red Bulls defenders Michael Amir Murillo and Fidel Escobar both started and went the full 90 for Panama in a 2-0 loss to Venezuela.
Murillo and Escobar returned to their National Team following their first World Cup appearance.
Venezuela got a brace from Salomon Rondon for the game-winner and insurance goal.
Murillo and Escobar's next match with Panama pits them against Japan on October 12.
